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What is the function of the liquid inside the virus sampling tube?

Author: Site Editor Publish Time: 2022-09-27 Origin: Site


Viruses are very tiny microorganisms that are made up of nucleic acids and protein shells and do not have their own metabolic machinery or protease system. Therefore, a virus cannot survive if it leaves the host cell. Such a tiny organism can bring us many diseases, such as the common epidemic infections usually RNA virus, influenza virus, HIV, and influenza A virus. Since it has no cellular structure of its own, the virus itself cannot replicate, but invades genes into the host cell and replicates new viruses with the help of the latter's replication system. After the virus sample is collected, the sampled swab is placed in preservation solution for storage and transport in order to maintain the activity of the virus sample and prolong the survival time of the virus in the sample.

 

Virus preservation solution (inactivation) inactivates the virus by denaturing the protein of fresh clinical virus specimens, eliminating secondary transmission of infection and ensuring the safety of transport and testing personnel. Inactivated samples can be used with a variety of virus extraction kits, nucleic acid extraction instrument for rapid extraction of nucleic acids, as well as with PCR kits for rapid detection of respiratory pathogens, and specificity and sensitivity are not affected.

 

Virus preservation fluid has many functions.

 

①, easy to operate, the liquid contains components that denature proteins, thus playing the role of virus inactivation and eliminating secondary infection.

 

②, containing inhibitors, a greater degree of protection of virus nucleic acid stability from degradation, significantly improve the nucleic acid extraction rate.

 

③, the product can be stored at room temperature for 12 months before sampling, and the sample can be stored at room temperature for one week after sampling, with good airtightness, which is convenient for preservation and transportation and saves transportation cost.

 

In addition to the inactivated virus preservation solution introduced above, we also have non-inactivated virus preservation solution. It contains Hank's liquid, potassium chloride, magnesium chloride, calcium chloride, BSA, etc. The combination of multiple antibiotics has anti-bacterial and anti-fungal effects; bovine serum protein (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus, making it less prone to decomposition and ensuring the integrity of the virus; the neutral environment constructed by Hank's buffer helps increase the survival time and infection stability of the virus. Inactivated and non-inactivated virus preservation solutions are usually used for the collection and delivery of virus specimens such as clinical influenza, avian influenza, hand, foot and mouth disease, measles, and specimens of mycoplasma, ureaplasma, chlamydia, etc.

 

Brilliance has been dedicated to the research and related production and sales of nucleic acid testing, molecular diagnostics, medical testing, and virus testing since 2008, and our products such as swabs and virus sampling tubes are highly regarded both at home and abroad!

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