Inactivated preservation solution is a kind of virus preservation solution, mainly used to inactivate the virus to prevent secondary infection, the characteristics of this virus preservation solution is able to quickly inactivate the virus, the virus protein membrane coat degradation off, while the virus nucleic acid release, the new crown virus is the release and degradation of RNA virus, so that you can carry out post-reverse transcription PCR amplification experiments, to achieve the purpose of using nucleic acid detection virus.

In addition to the lysis components, the inactivated virus preservation solution usually contains decontaminants that denature proteins and destroy membrane structures, biological buffers and inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain stable nucleic acid structures. The lysis system may also incorporate proteases that cut the protein into small fragments to facilitate the separation of the protein from the nucleic acid.

There are various methods of lysing viruses, the two generally more common being the heating method and the concentrated salt method.

The concentrated salt method uses a high concentration of salt that can break the secondary bonds between the nucleic acid and the protein, causing the nucleoprotein to unlink thereby releasing the viral nucleic acid. Usually guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt lysis salts are used, which can directly lyse and inactivate the virus without heating the sample, easy and fast

The heating method is based on the temperature value by heating to 80℃~100℃, because the samples are usually frozen at -70℃, and usually need to be heated for 5~10 minutes. This operation is relatively simple, and the template for nucleic acid PCR amplification can be obtained in just one step, without any centrifugation, extraction and other operational steps, which is faster than the commonly used CheleX100 extraction method and phenol/chloroform, etc. Although this method is simple and fast, the amount of nucleic acid extracted will be less