After nucleic acid collection, usually specimens are not immediately PCR detection, often need to be collected with specimens of swab samples for transport, and the virus itself in vitro will soon crack off affect the subsequent detection, so in the preservation and transport, it is necessary to add virus preservation solution for preservation, which is disposable virus sampling tube.
Single-use virus sampling tubes are usually divided into two types, non-inactivated and inactivated type. So how to choose between the two types of virus sampling tubes?
For different testing purposes need to choose a different virus sampling tube. The two types of virus sampling tubes are widely used have their own characteristics. In order to meet the different testing requirements, as well as different virus testing laboratory conditions, the correct choice of sampling tube is very necessary.
Inactivated virus sampling tubes.
Mainly nucleic acid extraction lysate modified virus lysis type preservation solution. Sampling contains a high concentration of guanidine salt can be highly efficient inactivation of the virus, can effectively prevent the operator secondary infection, but also contains Rnase inhibitor, can protect the virus nucleic acid is not degraded, so that the subsequent can be detected by NT-PCR. It can be stored at room temperature for a relatively long period of time, saving the cost of virus sample storage and transportation.
Non-inactivated virus sampling tubes.
It is mainly a virus maintenance fluid type preservation solution modified on the basis of transport medium. It preserves the in vitro activity of the virus and the integrity of the antigen and nucleic acid, protects the virus protein shell from decomposition, and maintains the originality of the virus sample to a greater extent. In addition to nucleic acid extraction and testing, this preservation solution can also be used for virus culture, isolation and antigen detection, etc. However, strict low temperature is required for prolonged storage after sampling.
Whether inactivated or non-inactivated, the virus sampling tube before sampling must be strictly inactivated and sterilized to ensure that there are no other microorganisms in the tube that could cause virus decomposition or other effects resulting in false detection after sampling. After the swab sampling, if the use of lack of quality collection tools or preservation solution, will affect the subsequent test results or even cause misdiagnosis. Therefore, it is important to choose virus sampling tubes produced by professionally qualified manufacturers.
After sampling is completed, it should be noted that
1、Prohibit direct contact with the preservation solution in which the virus sample has been placed
2. It is prohibited to moisten the swab with the preservation solution before sampling.
3, virus sampling tube is a one-time product, only for clinical virus specimen sampling, delivery, and preservation, beyond the intended use shall not be used.
4、Pay attention to the shelf life and whether the packaging is intact before using the product.
5, the collection of virus specimens should be carried out by professionals in strict accordance with sampling procedures; specimens into the test should be operated in a laboratory that meets the safety level.
6, after sampling must be marked clearly sampling information.
