Virus sampling tube sampling is mainly divided into oropharyngeal sampling and nasopharyngeal sampling.

1 . Oropharyngeal sampling: the tongue must be pressed against the tongue, and then the head of the sampling swab is placed into the throat to scrub both sides of the pharyngeal tonsils and the posterior pharyngeal wall, gently scrubbing the posterior pharyngeal wall with force, avoiding contact with the tongue.

2 . Nasopharyngeal sampling: measure the distance from the tip of the nose to the earlobe with a swab, mark it with a finger, insert the sampling swab into the nasal cavity along the straight nose (face) direction, put the swab in a distance of at least half the length from the earlobe to the tip of the nose, and swab 15-30 in the nose.

It is clear from the application of the method, oropharyngeal swab or nasopharyngeal swab, sampling is a technical work, difficult, easy to contaminate, the quality of the collected samples and direct follow-up examination, the collected samples are easy to be false negative if the amount of virus is low.

Nowadays, commercially available kits recommend the use of oropharyngeal swabs, nasopharyngeal swabs and alveolar washings. If venous blood samples are used, especially special nucleic acid test tubes to collect blood and extract refined RNA for examination, the operational difficulty of sampling personnel can be substantially reduced. Collecting venous blood samples is not difficult? And like examining hepatitis C RNA, about 5 ml of EDTA anticoagulated blood samples are separated from the plasma and the extracted purified RNA fully meets the needs of nucleic acid testing.