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Does Delta affect the new crown nucleic acid rapid test? How to extract pure nucleic acid?

Author: Site Editor Publish Time: 2020-05-24 Origin: Site

The outbreak is raging and the Delta variant strain is once again a cause for concern.

 

But the Delta+ variant is already spreading where we haven't even touched it yet. British experts say the Delta+ variant of the New Coronavirus may be more infectious than the Delta New Coronavirus variant.

In addition to "Delta+", a new variant of Delta virus "AY.4.2" is also spreading simultaneously.

 

According to current information, "AY.4.2" has already spread in the UK, US and Denmark. Although no studies have been done to show its ability to cause severe illness, this variant is about 15% more contagious than the Delta strain and has been called the "most evolved form" of the virus. Currently, WHO has classified AY.4.2 as a "beware" strain.

 

As the virus continues to mutate, questions have been raised about the accuracy of the New Coronavirus Nucleic Acid test in detecting the virus: will the virus mutation escape or affect the results of the New Coronavirus Nucleic Acid Rapid Test?

 

This starts with the New Crown Nucleic Acid Rapid Test, which is essentially a precision tracker that can pinpoint a hidden virus and report on its specific nucleic acid fragments. Since viruses typically hide in epithelial cells such as the respiratory tract, human cells need to be hydrolyzed with the proteins encasing the viral nucleic acid before performing the New Crown Nucleic Acid Rapid Test until the nucleic acid is fully exposed to ensure the accuracy of the New Crown Nucleic Acid Rapid Test.

 

How is that nucleic acid to be extracted? Currently the most important automated nucleic acid extraction generally uses the magnetic bead method of extraction, we talk about this method below.

 

1、Adding lysis buffer to the original sample, yes the cells are lysed and nucleic acid is released.

 

2, By using the magnetic bead material in different salt concentration and acid-base conditions to rain nucleic acid specific adsorption or dissociation, the nucleic acid is separated from the cell magazine, and the nucleic acid is transferred to the washing solution for several washes.

 

3. Finally, transfer to the elution solution, elute the nucleic acid from the magnetic beads, discard the beads, and obtain the pure nucleic acid.

 

After completing these operations, the nucleic acid extraction is completed. Getting pure nucleic acid is an important task in the rapid detection of new crown nucleic acid, which determines the accuracy of subsequent amplification and product analysis.

 

After the nucleic acid extraction is completed, it needs to undergo amplification to increase the pure nucleic acid exponentially to a certain concentration to make the reaction more sensitive.

 

There is already highly integrated automated equipment capable of extracting nucleic acid and constructing PCR systems that can extract high-purity nucleic acid and provide the basic conditions for subsequent amplification, freeing the hands of the inspector while significantly improving the efficiency of rapid testing of new crown nucleic acid.

 

In addition to this conventional process, there is also a new crown nucleic acid rapid test solution, just a simple extraction of nucleic acid, can be amplified on the machine, efficient amplification reagents with portable amplification instrument, to achieve the possibility of "instant detection" to meet the needs of different scenarios

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