Usage.
I: Use . Nasal Sampling Swab 50 sticks Instructions Fresh Tissue Sample
1. Estimate the volume of this product needed to completely submerge the sample (10 mL for 1 g of tissue).
2. Mark the collection tube and add the estimated amount of product required.
3. Cut the sample into pieces less than 0.5 cm thick as fast as possible.
Note: Small organ samples such as mouse liver, kidney and spleen and plant samples without wax protection layer can be stored in this product without shearing, while plant samples with wax protection layer need to have the wax skin broken first.
4. Completely submerge the tissue fragments in the collection tube of this product.
5. Store the collection tube at an appropriate temperature for no longer than the zui duration of storage at that temperature. For storage at -20°C or -80°C, leave the sample at 4°C overnight before transferring to the zui temperature. Note: Before transferring the sample to -20°C or -80°C, the protective solution needs to be discarded. Common storage temperatures are related to their zui duration as follows.
6. Remove the sample from storage (samples stored at -20°C or -80°C need to be melted at room temperature) and remove the tissue fragments from the protective solution using sterilized forceps.
Immediately start RNA extraction or other processing (e.g., splitting the sample into smaller pieces for re-preservation, etc.). Note: Samples can be repeatedly freeze-thawed up to twenty times without affecting the quality of the RNA.
Disposable Sampling Swab Instructions Step 1: Gently insert the sampling swab into the oral or nasal cavity by holding the handle. Step 2: Gently rotate the sampling swab 3-5 times and then slowly remove it. Step 3: Place the extracted sample into the sample collection tube, break the handle, and seal it up immediately to complete the sampling.
Why is it necessary to use nasopharyngeal swabs for patients suspected of having pertussis?
Because Bordetella pertussis is parasitic in the ciliated respiratory epithelium of the nasopharynx and has a mechanism to attract and attack ciliated epithelial cells and phagocytes in the respiratory tract and survive, and it is the main cause of pertussis. The isolation rate is higher.
