1、Uses of virus sampling tube?
For the collection, delivery and storage of virus samples such as influenza, avian influenza and coronavirus for subsequent extraction and amplification applications.
2、The difference between inactivated and non-inactivated preservation solution?
After the collection of virus samples, the general sample collection office can not be timely PCR detection, so the need for the collection of virus swab samples for transport, and the virus itself in vitro will soon be lysed and then affect the subsequent detection, so in the preservation and delivery, it is necessary to participate in the virus preservation solution, for different detection purposes, the need to use different virus preservation solution. At present, there are two main types of preservation solutions: inactivated and non-inactivated. In order to satisfy different testing requirements and different virus testing laboratory conditions, it is necessary to use different preservation solutions.
Inactivating preservative.
Biological preservation solution B can lyse the virus in inactivated samples, so that the virus loses its infectious activity. It effectively prevents secondary infection of the operator, but contains inhibitors to maintain the viral nucleic acid from degradation, which then allows subsequent detection by NT-PCR. It can be stored at room temperature for a relatively long period of time, saving the cost of virus sample storage and transportation.
Non-inactivating preservative.
Biological preservation solution A it can adhere to the in vitro activity of the virus as well as the integrity of the antigen and nucleic acid, maintain the virus protein shell from decomposition, and adhere to the originality of the virus sample to a greater extent. In addition to being used for nucleic acid extraction detection, it can also be used for virus culture, isolation, etc.
Biological virus sampling tube with thickened and leak-proof design, to ensure that the sample does not leak during the delivery process. It is a sampling tube that complies with WHO rules and biosafety rules.
3、How to collect samples?
According to the "Technical Expert Agreement on the Collection of Specimens from Pneumococcal Pneumonia Patients", the specific methods for collecting nasal and pharyngeal swab specimens are as follows.
Nasopharyngeal swab collection
1. The patient's head is tilted back (about 70 degrees) and held still.
2、Estimate the ear root to nostril interval with the swab stick.
3, from the nostril straight facial direction insert, deepen the interval should reach at least half of the length of the earlobe area to the tip of the nose. After encountering resistance that arrives at the posterior nasopharynx, should stay for several seconds to draw secretions (generally required 15-30s), should rotate the swab 3 to 5 times.
4, it is advisable to gently rotate to remove the swab, and the swab head is immersed in a collection tube containing 2mL of lysis solution or cell preservation solution containing RNAase inhibitor.
5、Break the sterile swab rod at the tip, discard the tail, screw the tube cap and close it with sealing film.
Oropharyngeal swab collection
1、Ask the patient to rinse the mouth with saline or water first.
2、Put the swab into sterile saline and moisten it.
3、Sit the patient down, tilt the head back, open the mouth wide and make the "ah" sound.
4、Fix the tongue with a tongue depressor, and swab over the tongue root to the posterior pharyngeal wall and tonsillar fossa, lateral wall, etc.
5, should first use the swab moderate force to scrub back and forth bilateral pharyngeal tonsils at least 3 times, and then the posterior pharyngeal wall at least 3 times, 3 to 5 times is appropriate.
6, Remove the swab to prevent touching the tongue, pendulous body, oral mucosa and saliva.
7, Immerse the swab head into the virus preservation solution containing 2 to 3 mL.
8、Break the sterile swab rod near the tip, discard the tail, screw the tube cap and close it with sealing film.
