Since the outbreak, nucleic acid testing has become more and more familiar to us, and many people have some understanding of the swabs used for nucleic acid testing, but little understanding of the virus tube that the swabs are put into after sampling.
Why is it necessary to put the virus sampling tube inside after nucleic acid collection?
After the nucleic acid collection, usually the specimen can not be immediately PCR detection, often need to be collected with the specimen swab samples for transport, and the virus itself in vitro will soon be cracked off affect the subsequent detection, so in the preservation and transport, it is necessary to add virus preservation liquid for preservation, which is disposable virus sampling tube.
Virus sampling tube liquid, also known as virus preservation fluid, is non-toxic, it can play a role in virus inactivation by denaturing the protein of fresh clinical virus specimens, eliminating secondary transmission of infection and ensuring the safety of transport and testing personnel. Virus preservation solution in virus sampling tubes is usually divided into two types, namely inactivated and non-inactivated.
1. Inactivated type.
Inactivated preservation fluid color usually has two kinds of red and white, there are a few manufacturers use purple. Inactivated preservatives are usually colored to make it easier to observe whether the preservative is leaking or not, as well as to make it easier for the laboratory to observe the surface of the preservative when it is drawn after sampling.
Inactivated virus preservation solution is mainly a modified nucleic acid extraction lysis solution, which contains a high concentration of lysis salts that can rapidly and efficiently inactivate the viral proteins in the sample to be tested, effectively preventing infection, and contains Rnase inhibitors that protect the viral nucleic acids from degradation, so that subsequent NT-PCR experiments can make a diagnosis as long as the viral nucleic acids can be detected. The subsequent NT-PCR test can make a diagnosis as long as the nucleic acid of the virus is detected. And can be stored at room temperature for a relatively long time.
2、Non-inactivated.
Non-inactivated preservation solution is usually red in color, and when the preservation solution deteriorates, its PH will change, so it is easy to identify whether the preservation solution has deteriorated. Non-inactivated virus preservation solution is mainly based on the transport medium as a modified virus maintenance fluid type preservation solution, can protect the virus protein shell and viral nucleic acid DNA or RNA, so that the virus in vitro with protein antigen epitopes and nucleic acid integrity, operation errors will have a certain risk of infection. Long-term storage after sampling needs to maintain strict low temperature.
The above is a brief introduction to the liquid in the nucleic acid virus sampling tube. Although the liquid in the tube is non-toxic, we know that many things are different from person to person, so try to avoid contact with the liquid in the nucleic acid virus sampling tube, in addition, if you encounter obvious leakage, you should provide feedback to the medical staff in a timely manner.
Note: Single-use virus sampling tubes are for in vitro diagnostic use and should not be used for internal or external use in humans or animals. If swallowed, it can lead to serious incidents; it is irritating to the eyes and skin, so if splashed in the eyes, rinse with water.
