Step 1: Use a disposable swab (pharyngeal/nasal swab) to detect the virus
New coronaviruses tend to "reside" in the respiratory tract of infected individuals. Sampling therefore requires deeper penetration into the pharynx (using a reagent stick to "rub" against the respiratory tract) to obtain a sample of the pathogen. Sampling can be divided into nasopharyngeal swabs and oropharyngeal swabs, depending on how they are inserted. Currently, oropharyngeal swabs are more widely used because they are more convenient and faster.
The key to sampling is to "rub" the right spot. The health care workers responsible for sampling nucleic acid testing have received rigorous professional training and are masters at dabbing samples.
Is it okay to eat before a nucleic acid test?
Relax, for nucleic acid testing.
Avoid eating? Not important! Feel free to eat snail noodles and garlic, 30 minutes before sampling, just don't touch cigarettes, alcohol and chewing gum.
Fasting? No need! This does not affect the test results, but if you are afraid of vomiting embarrassment, try not to eat 2 hours before sampling.
Cold? It doesn't matter! One size does not fit all, and the new coronavirus and the flu virus are not mutually exclusive.
Pain? It doesn't exist! Most people "don't feel anything" about it, and sensitive people only "feel a little discomfort".
On the day of the test, I woke up early with a lot of excitement. After eating fried durian with onions, I went to the test site and waited for a good-looking doctor to fill out the form, I took off my mask as instructed and "ahhh" -
Close your eyes, I feel ...... curious: not enough? Still going in? Suddenly, that reagent rod stopped at the back wall of my throat and turned! Couldn't resist! I'm going to vomit! I'm going to vomit! Too late, the reagent stick was drawn and suddenly put into the reagent tube, sealed up, the collection was also successful, vomiting was held back, but I was pearly -
In fact, the reagent stick "in" is only to ensure the accuracy of the test, which generally causes at most a slight foreign body sensation, but due to individual differences, I want to "vomit", not without ... ... ... not waiting for me to regain consciousness, the doctor even "after the sample is not recognized", and urgently greeted the "new love", which is not even half a minute to it! Hey, but I had no choice but to get up and give up my seat, mixed feelings back home and that has my test sample reagent tube, but also to go to complete its "atonement journey".
The second step, single-use virus sampling tube "trapped" virus
Before being transported to the nucleic acid testing center, the outer layer of the reagent tube will be covered with a layer of sealing material, a layer of foam material, and then sealed with a special transfer box, and finally add a lock to prevent the virus from leaking.
Not only to let the virus "can not run", but also to let it "can not die": the new coronavirus is a single-stranded RNA virus, if not cryopreserved for a long time, prone to nucleic acid degradation, affecting the nucleic acid test results. In short, the "escort" virus has two points: the temperature must be low! The speed must be fast!
Step 3: Use primers to "illuminate" the virus
After the sample is sent to a specialized nucleic acid testing center, the testers will sterilize it several times, remove the reagent tubes from the biosafety cabinet, and verify the information. In addition, due to the high risk of highly active viruses, in order to protect their own safety, while ensuring the accuracy of the test results, the testers will inactivate the virus in advance, so that the virus becomes a "broken-legged antelope", "good waiting for the test".
Once everything is ready, the primers with a "keen eye" will be added to the "mixed" respiratory pathogens samples to find the new coronavirus.
1. Inactivation of samples at 56°C prior to processing.
2. Registration of sample information.
3. Inactivated samples are placed neatly by number, shaken and mixed in preparation for nucleic acid extraction.
4. Add the nucleic acid template of the sample.
Huh? But whose virus is the virus? What if I get the wrong reagent tube? Don't worry, all the information will be entered by a person in charge, will ensure that "your results are yours".
After the test results come out, if positive, the Shenzhen CDC will review to ensure that "no wrongdoing a sample". After confirmation, the infected person should receive treatment at the designated institution, regardless of whether there are corresponding symptoms.
If the result is negative, "lucky goose" continue to blow air conditioning at home, unless ...... have similar symptoms and CT diagnosis, then you have to promptly consult a doctor, regular nucleic acid testing, to distinguish whether the disease is a new crown, or Mycoplasma pneumoniae and other symptoms similar to the disease ......
