I. Operation preparation
1. Protection preparation.
The collection personnel should wear a hat, double-layer latex gloves, isolation gown, one-piece protective clothing, N95 mask, goggles/protective mask, shoe covers, and waterproof boots in order according to the standard.
2. Preparation with materials.
1 test tube containing 3 ml of virus preservation solution, 2 plastic rod swabs with polypropylene fiber heads, tongue depressor, flashlight, self-sealing bag, black carbon pen, Aegis hand sanitizer, 75% alcohol spray solution, biosafety transport case.
II. Assessment
Patient's condition, age, degree of cooperation, check whether there are foreign bodies in the patient's mouth, and understand the patient's oral mucosa and pharynx.
III. Specimen collection
1. Prepare all materials for use and ensure the expiration date.
2. Check that the patient is correct, explain to the patient or family, and obtain cooperation.
3. Indicate the patient's information on the test tube: indicate the patient's name, sample type, collection time and other basic information.
4. Check whether there is any foreign body in the patient's mouth.
5. Press down the tongue root with the tongue depressor, remove 2 plastic rod swabs with polypropylene fiber heads, ask the patient to open his mouth to make the "ah" sound, and wipe the bilateral pharyngeal tonsils, posterior pharyngeal wall and palatal arch.
6. Dip the 2 swab heads into a test tube containing 3 ml of virus preservation solution, break the handheld portion and discard, tighten the lid, place vertically in a self-sealing bag, and seal tightly. 7. Spray both sides of the self-sealing bag with 75% alcohol. 8. Place the pharyngeal swab specimen in the yellow bucket inside the biosafety transport box, tighten the lid, and disinfect with 75% alcohol spray.
9. Cover the biosafety transport box, lock it, and disinfect it again with 75% alcohol spray.
10. After locking, the key is sprayed with 75% alcohol and put into the self-sealing bag, and the self-sealing bag is sprayed with 75% alcohol. Notify the property personnel to do a good job of personal protection (work cap, surgical mask, isolation gown, gloves) to send the biosafety transport box together with the key to the laboratory department, and notify the laboratory personnel to send the specimens to the CDC.
