Because of the epidemic, we can't go anywhere without nucleic acid testing. Many people only know that nucleic acid testing is the basis for checking new crowns, but they do not know what nucleic acid is? What is the key to nucleic acid testing?
What is nucleic acid.
Nucleic acid it is the polymerization of many nucleotides into biological macromolecular compounds, widely present in all animal and plant cells, microorganisms, nucleic acids in living organisms are often combined with proteins to form nucleoproteins. Depending on their chemical composition, nucleic acids can be divided into ribonucleic acid (RNA for short) and deoxyribonucleic acid (DNA for short). Also, since nucleic acids are unstable and easily denatured, we need to preserve them in appropriate ways to keep them from degradation or mutation. The new coronavirus is an RNA virus, and to do a good job of detecting the new coronavirus, it is inseparable from the product we talk about below - virus preservation solution.
Virus preservation solutions are divided into two categories: inactivated and non-inactivated virus preservation solutions. Their purpose is to protect viral nucleic acids from rapid degradation in vitro and to facilitate the amplification of specific nucleic acid sequences for detection purposes.
Principle of inactivated virus preservation solutions.
The main nucleic acid extraction lysis solution is a modified virus lysis type preservation solution, which has a high concentration of lysis salts added to it, which can rapidly and efficiently inactivate the viral protein of the sample to be tested, and can effectively prevent secondary infection of the operator, and at the same time contains Rnase enzyme inhibitors, which can protect the viral nucleic acid from degradation, and subsequent experiments can make a diagnosis as long as the viral nucleic acid can be detected. It can be stored at room temperature for a relatively long period of time, saving the cost of preserving and transporting virus samples.
Principle of non-inactivating virus preservation solution.
It retains both the protein shell of the virus and the viral nucleic acid DNA or RNA, which ensures the integrity of the viral sample nucleic acid during the delivery process. Although there is a certain risk of infection in case of operational errors, this preservation solution maintains the originality of the viral sample to the maximum extent and is a culture-based virus transport medium. It is important to note that prolonged storage after sampling requires strict cryopreservation.
Regardless of which virus preservation solution we use for nucleic acid testing, the main purpose is to quickly and effectively detect whether or not a virus is infected while ensuring the safety of the testers involved. This allows us to find out where the virus is hiding more quickly.
