Generally, it is better to store DNA in TE buffer for long-term preservation.

TE is Tris-HCl (PH 8.0) buffer containing EDTA: one of the more critical components is EDTA. DNase is inevitably present in the daily environment, and DNase activity depends on calcium ions and can be activated by magnesium ions or divalent manganese ions. DNase I can randomly shear double-stranded DNA at any site in the presence of magnesium ions.

In the presence of divalent manganese ions, DNase I can shear the DNA double strand at the same site, forming flat ends, or sticky ends with 1-2 nucleotides protruding. edta contained in TE can chelate metal ions and thus inactivate DNase. And the role of Tris-HCl is mainly to maintain a certain alkaline pH value, which helps to dissolve and stabilize DNA.

It is also possible to do without EDTA, and EDTA can affect the efficiency of downstream enzymatic digestion, ligation reactions and plasmid transfection into cells, so EDTA-containing buffers should be avoided if these experiments are to be done next.

Short-term storage of DNA can also be done with ddH2O, which can help with DNA ligation and other effects.

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