New Crown has been popular for more than two years, so I'm sure you are not unfamiliar with New Crown Nucleic Acid Rapid Test. But do you really understand all the aspects of the New Crown Nucleic Acid Rapid Test?
New Crown Nucleic Acid Rapid Test is more than just "sampling - on the machine - results" so simple. A rigorous Nucleic Acid Rapid Test should be performed in a negative pressure laboratory with multiple partitions, each with its own functional differences and pressure gradients to prevent biological contamination; and the complete Nucleic Acid Rapid Test process is "sampling - sample pre-treatment - nucleic acid extraction - nucleic acid amplification - result analysis. -Nucleic acid amplification - result analysis".
Today, we will talk about the "nucleic acid extraction" part of the New Crown Nucleic Acid Rapid Test process.
In New Crown Nucleic Acid Rapid Test, nucleic acid extraction refers to the complete lysis of the virus, the release of the virus RNA, through a series of washing and other operations, washing away impurities to obtain a high purity of nucleic acid.
And the quality and quantity of nucleic acid extraction and the accuracy of the nucleic acid test results in the latter area are greatly related. So how to get high purity and high yield of nucleic acid in the new crown nucleic acid rapid test? There are many methods of nucleic acid extraction in the market: magnetic bead method, column extraction method, etc. Today we will talk about the "magnetic bead method" first.
The magnetic bead method is used to extract nucleic acid by repeatedly washing the sample solution with magnetic beads to remove impurities and obtain nucleic acid.
The principle of magnetic bead method is that the magnetic bead material specifically adsorbs or dissociates from nucleic acid under different salt concentrations and acid-base conditions, and the nucleic acid and other components of the lysed cells are separated by transferring the magnetic beads, and then the nucleic acid is obtained by washing and elution of the extract without the magnetic beads.
The magnetic bead binding solution contains a powerful protein denaturant that rapidly dissolves the proteins and dissociates the nucleic acids; in its presence, the released nucleic acid components can be bound to the magnetic beads; subsequently, the proteins, inorganic salt ions and many organic impurities are removed by the action of the magnetic bead washing solution. The elution solution then elutes the pure nucleic acids.
Generally speaking, this method is characterized by large purity of nucleic acid, high yield of nucleic acid, good reproducibility of extraction, reliable quality, wide range of application, and high timeliness.
Of course, in addition to high quality nucleic acid extraction methods and reagents, obtaining high yield and high purity nucleic acid also has a great relationship with the nucleic acid extraction instruments. Currently, all nucleic acid extraction instruments on the market can be adapted to this extraction method, but some instruments can be used in conjunction with this method to obtain higher purity and higher yield of nucleic acid.
Dual lysis, dual module, equipped with PCR configuration function equipped with this extraction method, can obtain high yield and high purity nucleic acid at the same time shorten the new crown nucleic acid rapid detection time, greatly reducing the new crown nucleic acid rapid detection time, help the epidemic more precise, faster prevention and control.
