Disposable virus sampling tubes are centrifugal tubes for preserving samples after sampling, so do you know what the requirements for using virus sampling tubes are?
Sampling requirements for disposable virus sampling tubes.
Collected nasopharyngeal swab samples should be transported and examined immediately under 2~8. The sample transport and storage time should not exceed 48 hours.
Test methods for disposable virus sampling tubes.
1. Before sampling, disposable virus sampling tubes display relevant sampling information on the sampling tube label.
2. According to different sampling requirements, disposable virus sampling tube using a sampling swab to sample the nasopharynx.
3. The specific sampling methods of the disposable virus sampling tube are as follows.
a) Nasal swab: gently insert the head of the swab into the nasal palate in the nasal tract, stay for a while, and then slowly rotate it out. With another swab, wipe the other nostril and dip the head of the swab into the sampling solution.
b) Pharyngeal swab: With the pharyngeal swab, wipe both sides of the tonsils and the posterior pharyngeal wall, dip the head of the swab into the sampling solution and throw away the tail.
4. Quickly place the swab into the sampling tube.
5. Break the part of the sampling swab above the sampling tube and tighten the cap.
6. The newly collected clinical specimen should be transferred to the laboratory within 48 hours under 2~8.
Use of virus sampling tube.
1. Used for monitoring, sampling and application of infection pathogenic microorganisms in CDC and clinical departments. Applicable to influenza virus (common influenza, highly pathogenic avian influenza, influenza A (H1N1) virus, etc.) sampling. Hand, foot and mouth disease virus and other types of viruses. Also used for sampling of Mycoplasma, Chlamydia, Mycoplasma solium, etc.
2. Used for transporting nasopharyngeal swab specimens or site-specific configuration specimens from sampling sites to testing laboratories for PCR extraction and testing.
3. Preserve nasopharyngeal swab specimens or site-specific configuration specimens and perform the necessary cell culture.
Disposable virus sampling tube sampling process.
1. Keep the storage tube upright and unscrew the white cap on the storage tube.
2. (1) Swab sample: Insert the front end of the collected swab into the storage tube and break the front end of the swab into the storage tube along the indentation.
(2) Alveolar lavage fluid sample: Add 1 mL of alveolar lavage fluid to the tube.
3. Tighten the white cap and mix upside down and upside down 5 times.
4. The collected samples should be stored and transported under 37 conditions and are recommended to be used within one month.
1. Automatic extraction of reagents by magnetic bead method
(1) Turn on the power of the laboratory-assisted nucleic acid extractor.
After the instrument is started, the initial interface will appear on the instrument control screen.
(2) Gently tap the reagent strip on the table so that each reagent falls into the bottom of the test tube, place the reagent strip on the tray, and carefully tear off the sealing film.
(3) Add all samples to be tested to the first well on the left side of each reagent strip, then add 60 L DTT and 5 L booster solution.
(4) Place the tray on the corresponding area of the base and push the base into the instrument chamber. Then insert the magnetic bar sleeve into the groove of the bar sleeve holder; the bar sleeve should snap into place.
(5) According to the program selection guide interface, select the experiment running program "Virus RNA" and confirm the program name as the desired program. Click on the picture to start the program, and the instrument will automatically extract (please refer to the instrument manual for detailed steps). The instrument will sound the horn when it is finished.
(6) Remove the tray, the 10th hole from the left of each reagent strip is the eluted RNA, remove it with a pipette and put it into a centrifuge tube for spare.
2. Column manual extraction reagents
Since this preservation solution has the function of lysing virus, it is recommended that the step of pre-lysing virus can be omitted and as much sample solution as possible can be added directly for column extraction.
