Covid-2019 (novel coronavirus) is a virus in the coronavirus family, which consists of hundreds of viruses usually found in animals. According to research investigations, until today, there are 7 coronaviruses infecting humans, 3 of which are considered serious diseases, including the Severe Acute Respiratory Syndrome (SARS) virus that emerged in 2002, the Middle East Respiratory Syndrome (MERS) virus in 2012, and the novel coronavirus 2019.
It is reported that of the seven coronaviruses, the less serious ones only cause the common cold and flu, but the above three can cause a person to be unable to breathe and cause pneumonia, requiring hospitalization.
In fact, all three viruses belong to respiratory viruses. The more common respiratory viruses are coronaviruses, influenza viruses, syncytial viruses, rhinoviruses, parainfluenza viruses, adenoviruses, etc. They can be classified according to nucleic acid type, family classification, and whether they are accompanied by systemic symptoms as follows.
All three viruses are coronaviruses, which mainly invade the respiratory and digestive tracts and can involve the nervous system. Coronavirus is the most predominant pathogen of respiratory tract infections, accounting for about 15%-30% of the causative agent of the common cold.
In addition, all three viruses are RNA viruses and can be detected in the laboratory by using RT-PCR to determine whether the diagnosis is confirmed or the infected person by judging the Ct value.
RT-PCR (Reverse Transcription-Polymerase Chain Reaction) is a technique that combines reverse transcription (RT) of RNA and polymerase chain amplification (PCR) of cDNA. RT-PCR is a sensitive and versatile technique that can be used to detect the level of gene expression in cells, the content of RNA viruses in cells and to directly clone the cDNA sequence of a specific gene.
The PCR technique is performed by amplifying a specific fragment of the viral genetic material, which is referred to as the target in the assay process. We set the more stable fragment of the viral gene as the target gene by means of amplification to replicate the target gene exponentially, thus qualitatively analyzing the presence of the virus and determining whether it is infected or not.
In the rapid nucleic acid test for Neocrown, we used the N gene of Neocrown virus and ORF1ab gene as our target genes to design the test kit. Taqman fluorescent PCR technique was used to detect the N and ORF1ab genes of novel coronaviruses for the detection and diagnosis of neo-coronaviruses. The kit can be used for in vitro qualitative detection of novel coronavirus pneumonia in pharyngeal swabs and sputum samples from suspected cases, aggregated cases, and others who require diagnosis or differential diagnosis of novel coronavirus infection with a sensitivity of 500copies/mL.
The kits for the new coronavirus rapid nucleic acid test are suitable for application scenarios such as hospital testing departments at all levels, third-party testing centers, urban testing bases and large-scale screening, including but not limited to:
1) testing of existing confirmed patients in hospitals as well as inpatients and escorts;
2) testing of overseas import;
3) testing of return to work and school;
4) routine testing.
We add to the epidemic prevention and control and contribute to the strength.
