There are many companies in the market that promote inactivated virus preservation solution, saying that it has many advantages compared to non-inactivated type and is a better choice for virus sampling tubes. The answer is obviously no. Both have their own different characteristics and have different application directions.
Both types of preservation solution are necessary for different experimental purposes and different testing situations, which can be seen from the comparison of the two differences, although sometimes they can be used interchangeably, but need to adjust the experimental testing program according to the different types of virus preservation solution.
Inactivated virus preservation solution: It is mainly a modified virus lysis type preservation solution for nucleic acid extraction lysate, which has a high concentration of lysis salt added to it, which can rapidly and efficiently inactivate the virus protein in the sample to be tested, and can effectively prevent infection, and at the same time contains Rnase inhibitors, which can protect the virus nucleic acid from degradation, and subsequent NT-PCR experiments can be made as long as the virus nucleic acid can be detected. The diagnosis can be made as soon as the nucleic acid of the virus is detected in the subsequent NT-PCR test. It can be stored at room temperature for a relatively long time.
Non-Inactivating Preservation Solution: This is a virus maintenance solution based on the transport medium. This is a type that preserves both the protein shell of the virus and the viral nucleic acid DNA or RNA, so that the virus has the integrity of the protein antigen epitope and nucleic acid in vitro, but of course there is a certain risk of infection in case of mishandling. Prolonged storage after sampling requires maintaining strict low temperature.
From the two can be seen, the non-inactivated type is designed to retain the integrity of the virus protein, rather than some manufacturers claim that it is "incapable of extinguishing the virus", and its composition is more complex than the inactivated type, the cost is also slightly higher. As for the increased risk of infection, there is no need to worry, as long as the institutions that can do viral nucleic acid testing are regular and operate according to the requirements, there is no danger, and there have been no cases of virus infection caused by the virus preservation solution.
The non-inactivated virus preservation solution has higher experimental requirements, while its sensitivity is also higher and more applications, after all, the preservation solution added to the virus swab sampling tube is for virus detection services, can not be denied because of its infectivity, the real control of risk should be in the sampling and testing operations. Shenzhen Huarikang produces two different kinds of preservation solution, we recommend you to choose according to different needs, do not follow blindly, practice shall prevail!
