Single-use virus sampling tubes are usually divided into two types, a non-inactivated type, which protects the protein and nucleic acid of the virus, and an inactivated type, which usually contains the lysis salt of the inactivated virus, which lyses the protein and protects the nucleic acid.
Non-inactivated type.
The non-inactivated type of virus preservation solution preserves the virus with better integrity and higher detection rates, and can be used for other studies besides nucleic acid detection. Inactivated virus solutions are safer to use and require a less stringent operating environment, each with its own advantages.
Single-use virus sampling tubes (non-inactivated) are characterized by
Low temperature non-freeze type preservation, which does not destroy the shell of the virus and facilitates long distance transportation.
Suitable for a variety of swab samples, including oral swabs, nasal swabs, throat swabs, etc.
Can be used for H1N1 influenza virus and any other viruses that can be sampled with swabs.
Can be used to extract viral nucleic acids with column virus DNAout or column virus RNAout
The antibiotics in the sampling solution prevent bacterial and fungal contamination.
The sampling solution is supplemented with bovine serum albumin to protect the virus sample and improve the isolation rate.
Inactivated.
Virus preservation solution with added lysis salts is the inactivated type of virus preservation solution, which contains Tris, lysis salts, EDTA, etc. The main purpose is to release nucleic acids by lysing them, so that nucleic acid detection can be performed by subsequent real-time fluorescence RT-PCR to determine whether the sample contains nucleic acids characteristic of viruses, i.e. whether it is infected with viruses.
Nucleic acids are biological macromolecules composed of many nucleotides polymerized into two types of compounds: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which are among the most essential substances for life. Viruses have a single structure, containing only one nucleic acid and protein, so the detection of the characteristic nucleic acid is detected in addition to the virus. Viruses are parasitic organisms and cannot survive in vitro after sampling, so if they cannot be detected in time, they need to be placed in virus preservation solution. In order to protect the safety of the virus detection environment, it is necessary to add lysis salts to inactivate the virus and release the nucleic acid that can be detected.
Single-use virus sampling tube (inactivation type) features.
Easy to use, no liquid preparation, the system contains highly efficient virus lysis solution, immediately after sampling inactivate the virus, effectively prevent the risk of secondary infection, to protect the safety of transport and testing personnel;
Viral DNA/RNA can be stored and transported at room temperature for 1 week without degradation. After extraction according to most commercial kits, the DNA/RNA obtained is of good quality and high yield, which can complete various genetic testing and analysis experiments, such as PCR, qPCR, etc., while saving transportation costs;
Contains nucleic acid RNA enzyme inhibitor to maximize the protection of virus nucleic acid stability from degradation and greatly improve the efficiency of nucleic acid extraction.
It is suitable for the collection, preservation and transportation of common viral samples such as new coronavirus, influenza virus, hand, foot and mouth virus, etc. The samples can be collected from pharyngeal swabs, nasal specimens or specific parts of the tissue, and the stored samples can be used for subsequent clinical tests such as nucleic acid extraction or purification.
Precautions.
It is prohibited to directly contact the collected person with the preserved solution in which the virus sample has been placed.
It is forbidden to moisten the swab with the preservation solution before sampling.
This product is a disposable product and is intended for the collection, transport and storage of viral specimens for clinical collection only.
The product should not be used after the expiration date or if the product packaging is damaged.
Virus specimens should be collected by professionals in strict accordance with sampling procedures; specimens should be tested in a laboratory that meets safety standards.
Specimens should be transported to the corresponding laboratory within 3 working days after collection and stored at a temperature of 2-8 °C; if they cannot be sent to the laboratory within 72 h, they should be stored at -70 °C or below, and repeated freezing and thawing of specimens should be avoided.
