The thermostatic amplification chip method of New Crown Nucleic Acid Rapid Test is characterized by the design of 4 pairs of specificities for 6 regions of the target gene and the use of strand-switching DNA polymerase (Bst DNA polymerase) at constant temperature (around 65°C) through the non-cyclic start phase, the loop amplification phase, and the cyclic extension phase to finally form a series of products with multiple reverse repeats of target DNA in series without hearing the size of the product. The whole process can be completed in 15-60 min without heat deformation of template, temperature cycling, electrophoresis and UV observation.
The New Crown Nucleic Acid Rapid Test thermostatic amplification chip method is a combination of thermostatic amplification technology and microfluidic chip technology, which is characterized by the ability to detect multiple pathogens simultaneously in the same sample. The thermostatic amplification method is characterized by high integration capability, high automation, short detection time and low lower detection limit (up to 10²copies/mL). Compared with other rapid nucleic acid detection methods, the thermostatic amplification chip method is simple and rapid, less prone to contamination, and has low reagent consumption.
In contrast, the PCR technique uses the replicative properties of nucleic acids, and by adding markers, the nucleic acids are replicated exponentially in vitro using the markers, so that the replicated nucleic acids with markers reach a certain concentration and are reported positive. When the concentration of the marker does not increase, a negative result is reported.
When you see the above principle, you will think, since the new crown nucleic acid rapid detection of thermostatic amplification chip method can design multiple targets, and detection time is short, but also in the same sample to detect a variety of pathogens, then why the most used, and the most popular is still the PCR method?
This is related to the cost, the new crown nucleic acid rapid test of thermostatic amplification of the detection cost compared to PCR detection is much higher, and the current PCR application in the new crown nucleic acid rapid test technology continues to innovate, PCR amplification time has been reduced from the initial hours to the current several minutes, to achieve a multiplicative increase in efficiency.
In addition to the above two points, another one is that the whole process of New Crown Nucleic Acid Rapid Test has been fully automated, which frees the hands of New Crown Nucleic Acid Rapid Test personnel and greatly reduces the exposure risk of the test personnel.
Therefore, considering the cost, efficiency and safety, the PCR method is still our best choice.
The improvement of technology has created the automation of the whole process, currently our new crown nucleic acid rapid test generally contains several steps, in the sampling step due to the need for professional sampling personnel, while in the sample pre-processing, nucleic acid extraction, nucleic acid amplification interpretation analysis and other stages, there are already good auxiliary tools.
Future technology continues to improve, PCR technology for rapid nucleic acid detection of new crowns will still get leap forward, so PCR is still the mainstream methodology for rapid nucleic acid detection of new crowns at present.
