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The difference between inactivated and non-inactivated storage solutions

Author: Site Editor Publish Time: 2022-07-06 Origin: Site

①, the importance of virus preservation solution

 

In the process of nucleic acid testing, the role of virus preservation solution is particularly important, our test work can not be immediately after the end of the sampling test, from the completion of the sampling to the start of the test, it may happen in the middle of many things or encounter many problems, such as off-site transportation or mailing specimens, or too many samples and can not be tested on time, etc., when the virus preservation solution plays a considerable This is where virus preservation solution plays a significant role.

 

 

 

②. Why inactivated and non-inactivated preservation solutions

 

Virus samples are usually not collected in time for PCR, so the virus swab samples need to be transported, and the virus itself will soon be lysed in vitro to affect subsequent testing.

 

For different assay purposes, different virus preservation solutions are required, and each of these two types of preservation solutions is widely used. In order to meet different testing requirements, as well as different virus testing laboratory conditions, sampling different preservation solutions is very necessary.

 

 

 

③. Inactivating preservation solution

 

Mainly nucleic acid extraction lysate modified virus lysis type preservation solution. It can effectively prevent the operator from secondary infection, but contains inhibitors that can protect the virus nucleic acid from degradation, thus enabling subsequent detection by NT-PCR. It can be stored at room temperature for a relatively long period of time, saving the cost of virus sample preservation and transportation.

 

 

 

④. Non-inactivating preservation solution

 

It is mainly a virus maintenance fluid based on the transport medium. It can maintain the activity of virus in vitro and the integrity of antigen and nucleic acid, and contains Hanks' solution, etc. It protects the virus protein shell from decomposition and maintains the originality of virus samples to a greater extent. This preservation solution can be used for nucleic acid extraction detection, but also for virus culture, isolation, and antigen detection, etc. However, prolonged storage after sampling requires maintaining strict low temperature.

 

 

 

It can be seen that different types of preservation solutions have their own advantages and disadvantages depending on the situation, but it should be noted that: whether inactivated or non-inactivated, the virus sampling tube before sampling must be strictly inactivated sterilized to ensure that there are no other microorganisms in the tube, resulting in virus decomposition after sampling or other effects resulting in false detection.

 

After swab sampling, if you use a collection tool or preservation solution that lacks quality, it will affect the subsequent test results or even cause false positives of misdiagnosis, so it is recommended to use a good quality virus preservation solution.

 

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