Since the outbreak of the "novel coronavirus COVID-19", relevant diagnostic reagents R&D and manufacturing companies have launched RT-PCR nucleic acid detection kits in the first place, which can be used to collect samples from oropharyngeal swabs or nasopharyngeal swabs to extract and purify the RNA of the virus for qualitative or quantitative detection of " 2019-neocoronavirus". The sample collection and storage container used in this method is a "single-use virus sampling tube".
Composition of single-use virus sampling tube
Virus sampling tubes are medical devices that consist of a throat swab/nasal swab, a virus sampling tube (inactivated virus culture) / virus sampling tube (non-inactivated virus culture), and a specimen receiving bag. Since there is no unified national standard or industry standard, the products of various manufacturers vary greatly.
Pharyngeal swab: Shenzhen Hualikang Biomedical Technology Co., Ltd. single-use virus sampling tubes use flocked swabs for collection to enhance the positive rate of nucleic acid testing for new coronavirus.
Virus culture solution: consists of Hank's liquid base, gentamicin, fungal antibiotics, biological buffers and amino acids. It can maintain the virus activity in a wide temperature range, reduce the rate of virus decomposition and enhance the positive rate of virus isolation.
How to use the single-use virus sampling tube
Oropharyngeal sampling: first press the tongue with a tongue depressor, then extend the head of the sampling swab into the pharynx to wipe the bilateral pharyngeal tonsils and the posterior pharyngeal wall, swab the pharynx with mild force to avoid touching the tongue.
Nasopharyngeal sampling: measure the distance from the tip of the nose to the earlobe with a swab and mark it with your finger, insert the sampling swab into the nasal cavity in a vertical nose (face) direction, the swab should reach at least half of the length from the earlobe to the tip of the nose, make the swab stay in the nose for 15-30 seconds, gently rotate it 3-5 times and withdraw the swab.
The use of disposable virus sampling tube
1, used in the disease control department, clinical departments for infectious pathogenic microorganism monitoring sampling use. Suitable for influenza virus (common influenza, highly pathogenic avian influenza, influenza A (H1N1) virus, etc.), hand, foot and mouth virus and other types of virus sampling. Also used for sampling of mycoplasma, chlamydia, ureaplasma, etc.
2、Used for transporting pharyngeal swab specimens or tissue specimens of specific sites from sampling sites to testing laboratories for pending PCR extraction and testing.
3、Used to preserve pharyngeal swab specimens or tissue specimens of specific sites for necessary cell culture.
