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Some common questions about virus sampling tube
1、The use of virus sampling tube
For the collection, transportation and storage of influenza, avian influenza, coronavirus and other viral samples for subsequent extraction and amplification.
2、The difference between inactivated and non-inactivated preservation solution
Virus samples are usually not collected in time for PCR testing, so the collected virus swab samples need to be transported, and the virus itself will soon be lysed in vitro thus affecting subsequent testing, so when preserving and transporting, it is necessary to add virus preservation solution, and for different testing purposes, different virus preservation solutions need to be used. Currently, there are two main types of preservation solutions: inactivated and non-inactivated. In order to meet different testing requirements and different virus testing laboratory conditions, it is necessary to use different preservation solutions.
Inactivating preservative.
Warrican Biological Preservation Solution can lyse the virus in inactivated samples, causing the virus to lose its infectious activity. It effectively prevents secondary infection by the operator, but contains inhibitors that protect the viral nucleic acid from degradation so that subsequent detection by NT-PCR can be performed. It can be stored at room temperature for a relatively long period of time, saving the cost of virus sample storage and transportation.
Non-inactivating preservative.
Warrican Biological Preservation Solution it can maintain the activity of virus in vitro and the integrity of antigen and nucleic acid, protect the virus protein shell from decomposition, and maintain the originality of virus samples to a greater extent. In addition to being used for nucleic acid extraction detection, it can also be used for virus culture, isolation, etc.
Hualikon biological virus sampling tube has thickened and leak-proof design to ensure no leakage of samples during transportation. It is a sampling tube that meets WHO regulations and biosafety regulations.
3、How to conduct sample collection
According to the "Technical Expert Consensus on Specimen Collection from Patients with New Coronary Pneumonia", the specific methods for nasal and pharyngeal swab specimen collection are as follows.
Nasopharyngeal swab collection
1, the patient's head is tilted back (about 70 degrees) and kept still. 2, the distance from the root of the ear to the nostril is estimated with the swab stick, 3, the vertical facial direction from the nostril is inserted, and the depth distance should be at least half of the length from the earlobe area to the tip of the nose. After encountering resistance that reaches the posterior nasopharynx, it should stay for several seconds to absorb the secretion (generally required 15-30s), and the swab should be rotated 3-5 times. 4, it is advisable to gently rotate to remove the swab, and the swab head is immersed in a collection tube containing 2mL of lysis solution or cell preservation solution containing RNAase inhibitor. 5, the sterile swab rod is broken at the tip, and the tail is discarded, and the cap is screwed tightly and closed with sealing film.
Oropharyngeal swab collection
1、Ask the patient to rinse the mouth with saline or water first.
2、Put the swab into sterile saline and moisten it.
3、Sit the patient down, tilt the head back, open the mouth wide and make the "ah" sound.
4、Fix the tongue with tongue depressor and swab over the tongue root to the posterior pharyngeal wall and tonsillar fossa, lateral wall, etc.
5, should first wipe the bilateral pharyngeal tonsils back and forth with moderate force with the swab at least 3 times, and then the posterior pharyngeal wall at least 3 times, 3 to 5 times is appropriate.
6、Remove the swab to avoid touching the tongue, pendulous gland, oral mucosa and saliva.
7, Immerse the swab head into the virus preservation solution containing 2 to 3 mL.
8、Break the sterile swab rod near the tip, discard the tail, screw the tube cap and close it with sealing film.
