When the epidemic strain mainly belongs to Omicron BA.5 evolution once again waiting for the opportunity to move, in order to quickly control the spread of the virus, nucleic acid testing has become a necessary path, and also attracts the attention of all walks of life.
However, there are various factors that affect the results of nucleic acid testing, including sample collection site, collection method, storage and transportation process, nucleic acid extraction operation, and so on.
Among them, the stability of the sample is a key aspect. How to quickly inactivate the virus, but also to protect the virus nucleic acid from degradation, to maintain the stability of the test virus sample preservation solution has become the most important.
Shenzhen Huarakang Biomedical Virus Preservation Solution for New Crown Nucleic Acid Testing
The first step: break the shell and eliminate the "fighting force"
Like other RNA viruses, the results of the new coronavirus are simply composed of a protein shell wrapped around a single-stranded nucleic acid - RNA. We can simply think of the RNA of the virus as the "brain" that gives orders, and the proteins as the "limbs" that carry out the orders. The process of infection and reproduction of the new coronavirus, which causes disease, is mainly carried out by the biologically active protein structure outside the virus.
Inactivated virus preservation solution is mainly a nucleic acid extraction lysate modified virus; it is a powerful protein denaturant that rapidly dissolves proteins, causing the structure of the virus to break down. When a clinical sample with a new coronavirus is mixed with the preservation solution, the virus in the sample is subjected to the lysate and the protein shell is rapidly destroyed, and this destruction is irreversible. At this point, the virus loses its "minions" and no longer has the ability to infect people, thus ensuring the safety of those who come into contact with the sample during transport and handling.
Step 2: Inhibit enzymatic activity and escort "ID"
After the shell is cleaved, the RNA of the new coronavirus is free in the preservation solution. As a single-stranded macromolecule, RNA is not as stable as double-stranded DNA, and RNA enzymes, which are readily available in the natural environment, are responsible for hydrolysis and breakage of RNA. Therefore, viral RNA without shell protection is considered fragile and unstable, and needs to be stored at low temperature to ensure its integrity.
Step 3: Match extraction to identify the "real culprit"
The viral RNA is properly stored in the viral sample preservation solution, and a final step is required before testing: the RNA in the preservation solution is captured, concentrated, and added to the real-time fluorescent PCR system as the final sample for testing on the machine.
Ltd. produces virus preservation solution in addition to inactivated virus preservation solution, there is also non-inactivated virus preservation solution. It is to retain both the protein shell of the virus and the viral nucleic acid DNA or RNA, so that the virus in vitro has the integrity of the protein antigenic epitope and nucleic acid, but of course there is a certain risk of infectivity when the operation is not done correctly. Prolonged post-sampling storage requires maintaining strict cold temperatures. Regardless of the type of virus preservation solution, we need to follow the requirements for strict handling, storage or transportation when using it.
