Zhong Nanshan, a member of the Chinese Academy of Engineering, has made new progress in the research of nucleic acid detection of new coronavirus. The Guangzhou Institute of Respiratory Health said on the 22nd, the journal Clinical Infectious Disease published the results of a study by the team of Li Shiyue, vice president of the First Hospital of Guangzhou Medical University, the National Clinical Research Center of Respiratory Diseases, Guangzhou Institute of Respiratory Health and Ye Feng, chief physician of the hospital. This study was the first to develop pharyngeal rinse samples for nucleic acid testing of novel coronaviruses, and also compared with the detection rate of nasal swabs.
Nucleic acid testing is currently the primary test for new coronavirus infection, but studies have shown that different sampling specimens have varying sensitivities for nucleic acid detection of new coronaviruses.
Optimal sampling specimens should be consistent with.
1. non-invasive, convenient and easy to perform.
2. a high rate of positive viral nucleic acid detection
3. no contact with the patient during sampling
4. avoid aerosol biosafety issues as much as possible.
Currently, nasopharyngeal swabs are the most commonly used samples for nucleic acid detection of neocoronaviruses, and studies have shown that nasal swabs have a higher positive rate for nucleic acid detection of neocoronaviruses than pharyngeal swabs. However, patients generally experience significant discomfort with nasal swab sampling, and secondly, nasal swab sampling cannot avoid the problem of increased risk of infection to the sampler due to patient contact. There is a need to develop new, safe, convenient, and operable samples to detect the new coronavirus nucleic acid.
To address this problem, Shiyue Li and Feng Ye's team proposed a pharyngeal gargle sampling method, in which the posterior pharyngeal wall is shocked with sterile saline to obtain more epithelial cells and improve its positive detection rate. For this purpose, they collected both pharyngeal rinses and nasal swabs from patients with novel coronavirus pneumonia for nucleic acid testing, and compared the positive rates of both.
A total of 11 patients diagnosed with novel coronavirus pneumonia were included in the study, of which 6 patients with positive sputum nucleic acid virus tests were still hospitalized in isolation, and the remaining 5 patients had met the discharge criteria to enter the 14-day observation period. Nine cases were male and two cases were female, aged 26 to 83 years.
The average number of days between the onset of the disease and the time of sampling was (53±2) days. 6 cases with negative nasal swabs were tested positive for NNCV, while 17 cases with negative nasal swabs were tested negative for NNCV.
Currently, the global epidemic is severe, and pharyngeal rinse is a non-invasive, easy and effective sampling method for neo-coronavirus nucleic acid testing. Patients retain it for themselves to avoid contact with infected patients and reduce the risk of infection for medical staff. It can also reduce the waiting time for sampling, which is especially important for the current huge number of infected patients, limited medical staff and busy clinical work.
Source: China News Network
Guangzhou Daily All Media Editor Peng Jiao Shi
