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What's the difference between inactivated and inactivated virus sampling tubes?

Author: Site Editor Publish Time: 2020-08-18 Origin: Site

 

After nucleic acid collection, usually the specimen can not be immediately PCR detection, often need to collect specimens swab samples for transportation, and the virus itself will soon crack in vitro affect subsequent detection, so in the preservation and transportation, it is necessary to add virus preservation solution for preservation, this is the disposable virus sampling tube.

 

Single-use virus sampling tubes are usually divided into two types, non-inactivated and inactivated. So how to choose between the two virus sampling tubes?

 

Different virus sampling tubes should be selected for different detection purposes. The two virus sampling tubes widely used at present have their own characteristics. In order to meet different testing requirements, as well as different virus detection laboratory conditions, the correct selection of the required sample tube is very necessary.

 

Inactivated virus sample tube:

 

 

Mainly nucleic acid lysate modified virus lysate preservation solution containing high concentration of guanidine when sampling can effectively inactivate the virus, can effectively prevent operator secondary infection, but also contains Rnase inhibitors, It can protect the viral nucleic acid from degradation, so that the subsequent detection can be carried out by NT-PCR and can be stored at room temperature for a relatively long time, saving the cost of virus sample storage and transportation.

 

 

Non-inactivated virus sample tube:

 

 

It is mainly a modified virus maintenance solution based on the transport medium. It preserves the activity of the virus in vitro as well as the integrity of antigen and nucleic acid, protects the protein shell of the virus from decomposition,

To a large extent, the original nature of the virus sample is preserved. In addition to nucleic acid extraction and detection, the preservation solution can also be used for virus culture, isolation and antigen detection, but it needs to be kept at a strict low temperature for a long time after sampling.

 

 

No matter inactivated type or non-inactivated type, the virus sampling tube must be strictly inactivated and sterilized before sampling to ensure that there are no other microorganisms in the tube, which may cause virus decomposition or other effects after sampling to cause false detection. After swab sampling, if the use of poor quality collection tools or preservation solution, will affect the subsequent test results and even cause misdiagnosis.

Therefore, it is necessary to choose a virus sampling tube produced by a professional qualified manufacturer.

 

 

After the sampling is complete, note:

 

1. Do not directly touch the storage solution in which the virus sample has been placed

 

2. It is forbidden to infiltrate the swab with preservation solution before sampling

 

3. The virus sampling tube is a one-time product, which is only used for the sampling, transportation, and preservation of clinical virus specimens, and shall not be used beyond the intended use.

 

4. Pay attention to the shelf life and packaging before using the product.

 

5. Virus specimens should be collected by professionals in strict accordance with the sampling procedures; Specimens should be tested in a safe laboratory.

 

6. The sampling information must be clearly marked after sampling.

 

7. Pay attention to the preservation and transportation of specimens.

 

 

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