We all know that there are two types of virus sampling tubes, one is a virus maintenance preservation solution (non-inactivating preservation solution) modified on the basis of transport medium, and the other is a lysis-type preservation solution (inactivating preservation solution) modified by nucleic acid extraction lysate. Because the sampled samples may subsequently involve a variety of detection methods, inactivated preservation solution can only be used for nucleic acid extraction detection of viruses, while non-inactivated preservation solution can be used not only for virus culture, isolation and antigen detection, but also for nucleic acid extraction detection of viruses. However, it should be noted that no matter which preservation solution is used for sampling tubes, strict inactivation treatment should be performed before testing in order to ensure the safety of operators.
We tend to focus on the issue of nucleic acid degradation, which may be a pseudo-proposition or pseudo-need in response to this new crown outbreak. Nowadays, fever clinics require 6 hours or even 4 hours for test results, and nucleic acids barely have time to commit suicide. Even if the "willingness to test as much as possible" samples, and if you really need long-term storage, that -80 ℃ freezing is a more reliable way to meet basically.
Then how to choose the virus sampling tube? Preservation solution is easy to rot and spoil after a period of storage. If the clear preservation fluid starts to become cloudy, grow mold, bacteria, or the preservation fluid starts to change color, then congratulations, you bought a very poor quality virus sampling tube, medical equipment such things have always been a penny a piece, do not lose a lot because of greed for small. This change in physical properties visible to the naked eye implies that the preservation fluid has deteriorated. This kind of company that seriously affects the image of the company and the quality of the product is of course the first to pass away. After all, preventing contamination is no small challenge in the R&D, production and quality control process. Of course, it is not difficult to deal with, the workshop for more than 100,000 clean workshop, in the preservation pressure to add antibiotics to inhibit the growth of bacteria or can stabilize the buffer system and other initiatives, can avoid the growth of bacteria.
Some preservation solutions, open the box or packaging to see the tube leakage. The wall of the tube "drooling", the carton "bad", stained hands, laboratory bench, instruments, etc. everywhere, some visible, some invisible, which will make the experimental environment is vulnerable to contamination. Box transport process, brutal loading and unloading or by the negative pressure of air transport, it is easy to leak, the whole box and the whole box have to be scrapped. This domestic situation is okay, if it is a foreign order, return orders, returns, termination of contracts, etc. These are very tricky. Therefore, the sampling tube body and tube cover material selection, structural design, manufacturing process and other aspects of some effort, it is appropriate to use polyethylene - propylene polymer plastic and some special treatment of polypropylene (PP),
polycarbonate (PC) plastic and other containers, need to be airtight, high temperature and low temperature resistance, can be suitable for a variety of inactivation methods, and good negative pressure test.
Some sampling swab virus load is low, the fracture point is not suitable, the operator is not easy to break, etc., not only limit the efficiency of sampling, but also easy to cause the negative false nucleic acid detection. Therefore, the sampling swab head should use nylon fiber flocking is appropriate, should not choose cotton or containing calcium alginate sponge and other materials; swab handle material recommended to use hollow polystyrene (PS) extrusion tube or polypropylene (PP) injection indentation tube, do not use wooden sticks or bamboo sticks and other materials.
Because the swab containing calcium alginate and wooden components soaked in preservation fluid after fracture will adsorb protein, and even interfere with subsequent experiments, the fiber of cotton has a stronger adsorption of protein, which is not easy to elute; the length of the swab should not be too short, and the fracture position should not be close to the head of the swab, so as not to stain the patient's body fluids during operation; the location of the fracture point should be reasonable, with the sampling tube fracture, to avoid the liquid in the tube out of splash caused by The risk of contamination, but also afraid of improper break point suddenly in the sampling process broken in the human body caused by trauma.
Having said that, so you know how to choose a virus sampling tube?
