Nasopharyngeal sampling swabs are mainly used for nasal and pharyngeal sampling of respiratory and intestinal viruses such as influenza, swine flu, avian influenza, hand, foot and mouth. The use of nasopharyngeal sampling swabs should pay attention to the following realizations.

1, sampling objects should be within 3 days of onset and fever ≥ 38 ℃;

2, sampling fluid should be placed in a refrigerated box (put ice row), brought to the sampling site.

3, sampling tube capacity should be about 10ml, should not be too large, so as not to affect the post-processing of the specimen;

4、The sampling tube should be plastic tube to prevent accidents during transportation;

5, the nasopharyngeal swab rod is best to extend, easy to use when sampling;

6, the head of the nasopharyngeal swab should be made as small as possible to avoid discomfort to the patient's nose when sampling;

7, the liquid in the sampling tube should not be more than 4 ml, so as not to affect the concentration of the sample;

8、If necessary, double nasopharyngeal swabs can be collected as parallel samples;

9, the collected specimen should be immediately placed in a 4 ℃ refrigerator (if the site does not have a refrigerator, can be immediately put into a refrigerated box) within 24 hours with a refrigerated box transported to the laboratory for testing (at the same time, accompanied by a detailed sample registration form). If the sample cannot be delivered within 24 hours, it should be stored below -70℃;

10、When a case of pneumonia of unknown origin occurs, tracheal extracts can be collected.