Plastic-handled nylon swabs, polyester swabs, flocked swabs (which can absorb more biological samples and achieve a higher release rate) or rayon swabs (do not use cotton swabs with wooden handles because the latter may contain components that inhibit mycoplasma growth and inhibit molecular reactions)

Before collection, rinse the mouth with saline, ask the patient to tilt the head slightly, ask him/her to open the mouth and make the "ah" sound, use a tongue depressor to gently press the tongue surface if necessary to expose the pharynx, hold a sterile swab and wipe the secretions and epithelial tissue on the palatal arch, pharynx and tonsils on both sides with sensitive and gentle movements, wipe back and forth with slight force at least 3 times, then wipe up and down the posterior pharyngeal wall at least 3 times; after taking, insert the swab into a test tube, or put it into After taking, the swab was inserted into a test tube, or into a suitable preservation solution, the tube was tightly plugged, the time of specimen collection was indicated, and the specimen was sent for examination immediately. When eluting, dip the swab head into the sampling solution and place the swab head in contact with the tube wall several times to flush as much of the specimen as possible into the sampling solution. It is best to collect the specimen before treatment with antimicrobial drugs. The pharyngeal swab must be kept moist

Precautions for sample collection: Observe the patient's oral mucosa and pharynx for redness, swelling, ulcers, and purulent secretions, and let parents and patients know the collection site and purpose before collection, and obtain their positive cooperation