Performing specimen collection

According to the "Technical Expert Consensus on Specimen Collection from Patients with New Coronary Pneumonia", the specific methods for collecting nasal and pharyngeal swabs are as follows.

Nasopharyngeal swab collection

1, The patient's head is tilted back (about 70 degrees) and held still.

2、Estimate the ear root to nostril interval with the swab stick.

3, from the nostril straight facial direction insert, deepen the interval should reach at least half of the length of the earlobe area to the tip of the nose. After encountering resistance that arrives at the posterior nasopharynx, should stay for several seconds to draw secretions (generally required 15-30s), should rotate the swab 3 to 5 times

4, it is advisable to gently rotate to remove the swab, and the swab head is immersed in a collection tube containing 2mL of lysis solution or cell preservation solution containing RNAase inhibitor.

5、Break the sterile swab rod at the tip, discard the tail, screw the tube cap and close it with sealing film.

Oropharyngeal swab collection

1、Ask the patient to rinse the mouth with saline or water first.

2、Put the swab into sterile saline and moisten it.

3、Sit the patient down, tilt the head back, open the mouth wide and make the "ah" sound.

4、Fix the tongue with tongue depressor, and swab the tongue root to the posterior pharyngeal wall and tonsillar fossa, lateral wall, etc.

5, should first use the swab moderate force to scrub back and forth bilateral pharyngeal tonsils at least 3 times, and then the posterior pharyngeal wall at least 3 times, 3 to 5 times is appropriate.

6, Remove the swab to prevent touching the tongue, pendulous body, oral mucosa and saliva.

7, Immerse the swab head into the virus preservation solution containing 2 to 3 mL.

8、Break the sterile swab rod near the tip, discard the tail, screw the tube cap and close it with sealing film.