(1) For general bacteria, ocular secretions, especially purulent ones, were collected with sterile cotton swabs.
(2) For Chlamydia trachomatis, first wipe away the secretions above the conjunctiva, then scrape the fornix and conjunctival epithelium with a sterile knife, treat with streptomycin and set aside.
(3) For patients who have just been treated or drug-flushed, it is best to collect specimens after 12-24 h.
(4) For patients with dacryocystitis, specimens can be collected with sterile cotton swabs after slightly squeezing.
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