1、Reproductive tract specimens

The normal human internal genital tract is sterile, and the external genital tract and urethral orifice have normal flora present.

Reproductive organs are open organs, specimen collection should be strictly followed in aseptic operation. Vaginal and cervical specimens should be collected under a speculum, without touching the mucosa of the vaginal wall as much as possible.

Neisseria gonorrhoeae is weak and autolytic, so it should be sent for examination immediately after collection, or placed in a transport swab for 1 h.

Chlamydia is an intracellular parasite and the specimen must contain epithelial cells. Therefore, the specimen should be collected at the migrating epithelium of the cervix or 3-5 cm from the urethral orifice of the internal urethra for a few tens of seconds, turn and wipe the inner wall epithelial cells urethral secretions and urine is not suitable for isolation of chlamydia.

Mycoplasma is sensitive to heat and drying, and it is appropriate to send the material immediately after sampling.

Male genital tract specimen: flip the foreskin, wash the urethral orifice with soap and water, rinse with water and collect urethral secretions

Collection of prostatic fluid: rinse the urethra and bladder, massage the prostate to overflow prostatic fluid

Place the specimen on a sterile swab, sterile tube

at room temperature and sent for examination immediately

If the specimen cannot be sent in time, place it in a transfer swab and send it for examination within 1 hour

Normal human internal genital tract is sterile, but there are normal flora in the external genitalia and urethra of both sexes.

The detection of Enterobacteriaceae, Staphylococcus aureus and Enterococcus in the external genital tract is not clinically significant, while the internal genital tract can be considered as pathogenic bacteria

Group B Streptococcus and Listeria are clinically significant in vaginal and perineal specimens of the mother

Therefore! Application form specimen information is important, secretions or pus must indicate the collection site

2、Fecal rectal swabs

1, specimen collection pus and blood, mucus part of the stool 1-3 grams, liquid stool to take 1-3ml

2, sterile container or transfer swab within 2 hours of delivery

3、Vibrio culture specimen placed in alkaline peptone water for transportation