There are two types of single-use virus sampling tubes on the market today, one is non-inactivated, which protects the protein and nucleic acid of the virus, and the other is inactivated, which usually contains the lysis salt of the inactivated virus, which lyses the protein while protecting the nucleic acid. Let's talk about what is the difference between these two types of virus sampling tubes.
Non-inactivated virus tubes have better virus integrity and higher detection rates, and can be used for research other than nucleic acid detection. Inactivated virus storage solution is safer to use and requires a less stringent operating environment, each with its own advantages.
Non-inactivated virus sampling tubes can be preserved in low temperature non-freezing type, also will not damage the shell of the virus, convenient for long-distance transportation; also suitable for a variety of swab sampling samples, including oral swabs, nasal swabs, pharyngeal swabs, etc.; can be used for H1N1 influenza virus and any other viruses that can be used for swab sampling, non-inactivated preservation solution in the antibiotics can effectively prevent bacterial and fungal infections, preservation solution with the addition of bovine The antibiotics in the non-inactivating preservation solution are effective in preventing bacterial and fungal infections, and the addition of bovine serum albumin to the preservation solution protects the virus sample and improves isolation rates.
What is the difference between sterile and non-sterile virus sampling tubes?
Inactivated virus preservation solution contains components such as lysis salts, the main purpose of which is to lyse nucleic acids and release them for subsequent nucleic acid detection by real-time fluorescence RT-PCR to determine whether the sample contains nucleic acids characteristic of viruses, i.e., whether it is infected with a virus.
Nucleic acid is a biological macromolecular compound formed by the polymerization of many nucleotides, including both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and is one of the most essential substances for life. Virus structure single, it contains only a nucleic acid and protein, so the detection of the characteristic nucleic acid to detect the virus. Viruses are parasitic organisms and cannot survive in vitro after sampling, so if they cannot be detected in time, they need to be placed in virus preservation solution. In order to protect the safety of the virus detection environment, it is necessary to add lysis salts to inactivate the virus and release the nucleic acid that can be detected.
Features of the disposable virus sampling tube (inactivated type).
①, easy to operate and use, no liquid preparation, the system contains highly efficient virus lysis solution, inactivate the virus immediately after sampling, effectively prevent the risk of secondary infection, to protect the safety of transport and testing personnel;
②, virus DNA/RNA can be stored and transported at room temperature for 1 week without degradation, after extraction according to most commercial kits, the DNA or RNA obtained is of good quality and high yield, which can complete various genetic testing and analysis experiments, such as PCR or qPCR, etc., while saving transportation costs;
③, contains nucleic acid RNA enzyme inhibitor, to maximize the protection of virus nucleic acid stability from degradation, significantly improve the efficiency of nucleic acid extraction. It is suitable for the collection, preservation and transportation of common viral samples such as new coronavirus, influenza virus, hand, foot and mouth virus, etc. The samples can be collected from pharyngeal swabs, nasal swabs or specific parts of the tissue, and the stored samples can be used for subsequent clinical tests such as nucleic acid extraction or purification.
