I. Purpose: To take a specimen of secretion from the patient's pharynx for nucleic acid testing or bacterial culture.

II. Implementation points.

1. Assessment of the patient.

①. Understand the patient's condition, oral mucosa and pharyngeal infection.

②. Explain to the patient and obtain cooperation.

2. Operation points.

①, check the medical advice and prepare.

②. Ask the patient to rinse the mouth with water, then ask the patient to open the mouth and make the "ah" sound, and use the tongue depressor if necessary.

Remove the disposable sampling swab and gently and quickly wipe both palatal arches, tonsils and the posterior pharyngeal wall three times.

④, put the swab head vertically to avoid touching the tube wall contamination, forcefully break the tail of the swab at the mouth of the sampling tube bottle, discard the tail, tighten and cover the tube cap (strictly enforce aseptic operation)

⑤, after sampling again and the name of the patient.

⑥. Indicate the time of specimen retention and send it for timely examination.

Precautions to be noted.

①, swab into the tube should be placed vertically, do not wipe to the mouth of the bottle to avoid contamination. When put into the transfer box should be placed vertically to avoid leakage.

②, it is best to send / send to the laboratory on the day of sampling for testing.

③, before sending the test must be appropriate specimens and send the test form consistent, the appearance of the sampling tube must be clearly named basic information, no basic information specimens can not be tested; send the specimen is required to submit (or submit in advance electronic version) screening information form, send the test form and other materials, the submitted materials should ensure not to be contaminated, do not put together with the specimen.

④, sampling should be avoided within 2h after the patient has eaten in order to prevent dissection of the chart, and the collection action should be gentle, steady and agile to prevent causing discomfort to the patient

⑤. The pharyngeal swab should not touch other parts during the collection process to ensure the accuracy of the retained specimens.

⑥、Do not use antibacterial drugs when deciding to do pharyngeal swabs

⑦. The depth of pharyngeal swab collection and the length of mucosal contact time. When collecting a pharyngeal swab, if the sampling time is insufficient or inferior swabs are used because the subject has a greater reaction to vomiting, this may result in a false negative and lead to delayed treatment of the patient.