Due to the impact of the epidemic, the New Crown Nucleic Acid Rapid Test has become something we are very familiar with, and as we become more familiar with it, we also know more about it and understand it more deeply. We also know that there are "false positives, false negatives, and single positives" in the Nucleic Acid Rapid Test.
There were many speculations about the accuracy of the Nucleic Acid Rapid Test, but many facts have been verified that the Nucleic Acid Rapid Test is valid and accurate.
So, today we will talk about what behaviors can affect the accuracy of the New Crown Nucleic Acid Rapid Test?
First, let's talk about the situations that may affect the sampling steps of the New Crown Nucleic Acid Rapid Test process that cannot be sampled accurately.
1, before sampling, drink a lot of water. When the person being tested in a large amount of water before sampling, may be due to the flushing of water resulting in the pharyngeal virus concentration is too low, in the sampling of new crown nucleic acid rapid test can not collect the nucleic acid of the new crown virus, resulting in errors in the results of the new crown nucleic acid rapid test.
2. Before sampling, eat within two hours. We are currently the most commonly used sampling method is pharyngeal swab sampling, if the sampling 2 hours before eating, there may be food residues left in the oral pharynx, residues will be covered by the epithelial cells of the pharynx, the collection failed to pick up qualified samples.
3, the same, half an hour before the collection, smoking, drinking, chewing gum will make the pharynx virus concentration is reduced, it is likely to affect the new crown nucleic acid rapid test results.
However, there is one thing that you must not know: oral health can also affect the accuracy of the new crown nucleic acid rapid test. Japanese studies have shown that patients with poor oral health continue to test positive for viral NICs long after they have recovered.
Next, we talk about the conditions that can lead to errors during sample pre-processing.
Before the extraction of nucleic acid, the sample is opened, taken, closed and other actions, because a new crown nucleic acid rapid test personnel need to operate dozens or even hundreds of samples at the same time, in the completion of the above operations, it is likely to produce cross-contamination, resulting in a "false positive" situation. In addition, errors in entry information may result in a mismatch between the person and the sample, which can lead to misleading overall results for all samples.
Fortunately, most manufacturers have now introduced automated cup handling systems for sample pre-processing, which can effectively address cross-contamination due to improper handling by laboratory personnel.
Finally, let's talk about the behaviors that may lead to errors during amplification.
New crown nucleic acid rapid test needs to go through several stages, and the most critical one is the amplification stage, which needs to allow the target nucleic acid to increase exponentially and raise the concentration of nucleic acid in the sample for fluorescent labeling. However, if the amplification instrument is contaminated with spills or residual contamination from the previous Nucleic Acid Rapid Test, it can lead to a "tailing" of a curve in the Nucleic Acid Rapid Test, resulting in a "false positive" or "false negative". "False negative".
