Nucleic acid testing for new infections is essential to prevent the spread and progression of outbreaks. In the past round of nucleic acid testing, the "mixed test" method of nucleic acid testing has been widely debated. What is the significance of the "mixed test" method? Are the results of "mixed" nucleic acid tests reliable? Is there a requirement for virus storage solution for "mixed test"? These are all questions that we are concerned about?
What is a "mixed test" for nucleic acid testing?
A "mixed test" is a mixed sample nucleic acid test. It has two modes: one is to sample several people, such as three or five people, and then put them into the same sampling tube, this mode is also called "mixed sampling" or "mixed collection"; the other is in the laboratory testing, the samples of three or five people are mixed together in the same volume. The other is to take the same volume of samples from three or five people and mix them together in a laboratory test, also known as "sample mixing".
What is the significance of mixed sample nucleic acid testing?
Mixed test using 3:1 sample mixed test or 5:1 sample mixed test, compared to a single test, greatly improve the efficiency of detection, speed up the detection time, effectively enhance the detection capacity, which is conducive to the realization of "should be detected, willing to detect all".
"Mixed test" results are reliable?
How to protect the accuracy of the results?
From a scientific point of view, the first "mixed" mode, will not affect the sensitivity of nucleic acid detection, the latter will be mixed samples testing mode, the sensitivity of the test has a certain impact, but the degree of impact is known.
However, in the mixed collection, special attention should be paid to the registration of personnel information to ensure traceability between the sample number and the object being tested. Each place according to their own conditions, the use of single swab sampling and double swab sampling at the same time two ways to ensure the accuracy of the test results.
"Mixed test" on the virus sampling tube in the virus preservation fluid requirements?
Virus preservation fluid is divided into: inactivated and non-inactivated virus preservation fluid. The choice of virus preservation fluid can be based on the actual needs, this does not affect. However, when performing a "mixed test", whether it is a 3:1 sample mix or a 5:1 sample mix, the point is that the virus preservation solution should submerge the collected sample so that the virus preservation solution in the sampling tube does not have better access to the collected sample.
Mixed sample nucleic acid detection technology has significantly improved the ability to detect nucleic acid, reduce the cost of detection, is an important tool for the normalization of epidemic prevention and control. Ltd. made great efforts to develop virus preservation solution at the beginning of the epidemic, and was recognized for its quality or yield, whether inactivated or non-inactivated virus preservation solution.
